bis palmitoyloxy propyl  (InvivoGen)

Journal: Nature Communications

Article Title: Mechano-crosstalk between living and artificial cells

doi: 10.1038/s41467-025-63581-1

Figure Lengend Snippet: a Scheme of the process leading to Art-P cell construction. Pam3CSK4 is covalently attached to the membrane before template removal. SA is subsequently trapped inside to adjust rigidity. b Surface roughness plots of soft, medium, and rigid air-dried polysaccharidosomes before (blue) and after (yellow) Pam3CSK4 attachment (data are mean ± s.d. from each field (n); ≥50 artificial cells; n = 24 for soft, 19 for medium, and 23 for rigid polysaccharidosomes; n = 40 for soft, 34 for medium, 29 for rigid Art-P cells). ns P > 0.05, **** P < 0.0001. c Confocal microscopy images of Art-P cells following surface functionalization of polysaccharidosome membrane (green) with TLR agonist (Pam3CSK4, purple, labeled by 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD)). Scale bars = 10 µm. d AFM images of air-dried soft, medium, and rigid Art-P cells (scale bars = 10 µm). e Plots showing relative fold increase of the gene expression levels of pro-inflammatory cytokines (tumor necrosis factor α, TNF-α; chemokine (C-C motif) ligands 4, CCL4; interleukin 1β, IL-1β; inducible nitric oxide synthase, iNOS) by macrophages incubated with Art-P cells relative to the control sample (PBS) as analyzed by RT-qPCR, three biological replicates. f Plots showing the percentage of CD86 positive macrophages (four biological replicates), **** P < 0.0001, and g NO 2 - concentration in culture medium from macrophages that are co-cultured with PBS or Art-P cells (PBS, seven biological replicates; Art-P cell groups, eight biological replicates), **** P < 0.0001. h Mean fluorescence intensity of RITC-phalloidin labeled F-actin of macrophages (four biological replicates), and i quantification of actin anisotropy after co-culture with PBS or Art-P cells ( n = 25 for PBS, 44 for soft, 29 for medium, and 34 for rigid, three biological replicates). For e – i , data are mean ± s.d. j Confocal microscopy images of macrophage (blue) F-actin (red) morphology after co-culture with PBS or Art-P cells (scale bars = 10 µm). In b , e–i significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test. Source data are provided as a Source Data file.

Article Snippet: The as-prepared HA-NH 2 coated templates were modified by toll-like receptor 1/2 agonist Pam3CSK4 (N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-[R]-cysteinyl-[S]-seryl-[S]-lysyl-[S]-lysyl-[S]-lysyl-[S]-lysine, InvivoGen) before being incubated with EDTA.

Techniques: Membrane, Confocal Microscopy, Labeling, Gene Expression, Incubation, Control, Quantitative RT-PCR, Concentration Assay, Cell Culture, Fluorescence, Co-Culture Assay, Comparison

Journal: BMC Veterinary Research

Article Title: Mesenchymal stromal cells modulate infection and inflammation in the uterus and mammary gland

doi: 10.1186/s12917-023-03616-1

Figure Lengend Snippet: Activation of inflammatory response in endometrial and mammary epithelial cells. Bovine endometrial cell line (BEND) and mouse mammary alveolar epithelial cell line (Eph4) were transduced with luminescence NF-kB reporter ( A - B ). Activation of NF-kB, the master regulator of inflammation, in BEND cells is demonstrated in response to pathogen associated molecular patterns (PAMPs); LPS ( C ), PAM3CSK4 ( D ) and poly I:C ( E ). Activation of NF-kB in Eph4 cells in response to LPS and PAM3CSK4 is demonstrated ( F ). D-luciferin was added at time 0, 50 and 100 min (broken lines) and luminescence signals were quantified every 5 min using SpectraMax i3x multiple detection microplate reader (Molecular Devices, CA USA). Low resolution luminesce imaging of transduced BEND cells ( B ) was performed using IVIS Lumina Series III (PerkinElmer Inc., MA, USA)

Article Snippet: LPS (from E. coli serotype O55:B5, Sigma, Rehovot, Israel), Pam3CSK4 (((S)-(2,3-bis(palmitoyloxy)-(2-RS)-propyl)-N-palmitoyl-(R)-Cys-(S)-Ser-(S)-Lys4-OH-3HCl, 06B24-MT; InvivoGen, CA, USA) or poly I:C (Poly(I:C) HMW; cat. # tlrl-pic-5, InvivoGen, CA, USA) were used for cell activation as specified in the result.

Techniques: Activation Assay, Transduction, Imaging